Waters, HPLC
Refurbished Waters® Isocratic HPLC System
For laboratories that run straightforward, single-solvent separations day after day, a gradient system is often more than what the work demands. The Waters Isocratic HPLC System is purpose-built for exactly these applications delivering consistent, reproducible results with a simpler operational setup and a lower cost...
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Product Details
For laboratories that run straightforward, single-solvent separations day after day, a gradient system is often more than what the work demands. The Waters Isocratic HPLC System is purpose-built for exactly these applications delivering consistent, reproducible results with a simpler operational setup and a lower cost of ownership. At Spectrachrome, we supply fully refurbished and performance-verified Waters isocratic HPLC systems, making professional-grade chromatography accessible to labs working within a defined budget.
What Is an Isocratic HPLC System?
An isocratic HPLC system operates with a fixed mobile phase composition throughout the entire run. Unlike gradient systems that blend multiple solvents in varying ratios, the isocratic pump delivers one mobile phase at a constant flow rate and pressure from start to finish.
This makes isocratic HPLC the preferred choice for:
• Methods where the target analyte separates cleanly under constant solvent conditions
• Routine quality control testing with well-established, validated methods
• Laboratories running high sample volumes on a single standardized method
• Training environments and academic labs where method complexity needs to stay low
The Waters isocratic pump platform, particularly models like the Waters 515 HPLC Pump, has been a staple in pharmaceutical QC and academic research labs for over two decades due to its reliability and ease of use.
Key Technical Specifications-
| Feature | Specification |
| Pump Type | Single-channel isocratic pump |
| Flow Rate Range | 0.001 – 10.000 mL/min |
| Operating Pressure | Up to 6,000 psi (414 bar) |
| Flow Rate Accuracy | ±1.0% |
| Flow Rate Precision | <0.1% RSD |
| Wetted Materials | Stainless steel, PEEK, sapphire |
| Communication | RS-232, contact closure |
| Software Compatibility | Empower, Breeze, third-party data systems |
What Makes This Refurbished Unit Reliable?
Every refurbished Waters isocratic HPLC system supplied by Spectrachrome is reconditioned through a structured technical process. We do not simply clean and relist instruments — each unit is evaluated and restored to functional specification before dispatch.
Our refurbishment process includes:
• Pump head inspection and seal replacement to eliminate flow inconsistencies and pressure drops
• Check valve cleaning and verification for consistent priming and stable baseline performance
• Flow rate calibration at multiple points across the operating range
• Pressure leak testing at rated operating pressure
• Detector and communication port verification to confirm compatibility with data acquisition sys
• Full functional run using reference standards to confirm retention time reproducibility.
Ideal Applications-
| Pharmaceutical QC | Assay and dissolution testing for finished dosage forms where Ph. Eur. and USP methods specify isocratic conditions. |
| Academic Research | Teaching laboratories and university research groups where budget constraints make a refurbished isocratic system the most practical entry point into HPLC. |
| Chemical & Industrial Labs | Purity testing, raw material analysis, and process monitoring workflows that rely on established isocratic protocols. |
| Environmental & Food Testing | Residue analysis and additive quantification using validated isocratic methods under regulatory frameworks. |
PAN India Delivery & Technical Support
Spectrachrome delivers refurbished HPLC systems across India, including Delhi, Mumbai, Hyderabad, Pune, Chennai, Bengaluru, Kolkata, and Ahmedabad. Our team provides commissioning support and can assist with initial method setup and system suitability testing on delivery.
We also supply:
• Replacement pump seals and check valves for the Waters 515 isocratic pump.
• Compatible UV and PDA detectors for expanding system capability.
• Annual Maintenance Contracts (AMC) for ongoing technical support.
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