Agilent, HPLC
Refurbished Agilent® 1100 Series HPLC System
Refurbished Agilent 1100 Series HPLC SystemThe Refurbished Agilent® 1100 Series HPLC System is the global benchmark for high performance liquid chromatography. Renowned for its exceptional modularity, robustness, and analytical precision, the Agilent 1100 has served as the industry standard in pharmaceutical, environme...
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Product Details
The Refurbished Agilent® 1100 Series HPLC System is the global benchmark for high performance liquid chromatography. Renowned for its exceptional modularity, robustness, and analytical precision, the Agilent 1100 has served as the industry standard in pharmaceutical, environmental, food, and research laboratories worldwide. Professionally refurbished and certified by Spectra Chrome India using OEM Agilent parts, this system is tested to original factory specifications and supported with a warranty.
Available in isocratic, binary, or quaternary pump configurations, the Agilent 1100 supports a comprehensive array of detectors — including Variable Wavelength (VWD), Diode Array (DAD), Fluorescence (FLD), and RefractiveIndex(RID)—and is controlled via Agilent ChemStationor OpenLab CDSsoftware.Its forward-compatible design ensures compatibility with newer Agilent 1200 and 1260 modules and columns.
How the Agilent 1100 HPLC Works
Step 1: Solvent Degassing
The 4-channel vacuum degasser (G1322S) continuously removes dissolved gases from up to 4 solvents at up to 10 mL/min total flow, ensuring a stable, bubble-free mobile phase for excellent baseline performance.
Step 2: Solvent Delivery
The quaternary pump (G1311A) uses a high-speed proportioning valve and pre-configured vacuum degasser for low-pressure mixing of up to 4 solvents. The binary pump (G1312A) uses dual floating pistons in series for pulse-free high-pressure binary mixing from 0.001 to 5 mL/min.
Step 3: Sample Injection
The G1329A autosampler draws 0.1–100 µL aliquots from up to 100 sample vials and injects them into the mobile phase stream with minimal carryover (<0.1% typical) and high injection precision for accurate quantification.
Step 4: Column Separation
The sample passes through the temperature-controlled column compartment (G1316A), which uses Peltier heating/cooling to maintain column temperature from 10°C below ambient to 80°C. Stable temperature ensures consistent retention times.
Step 5: Detection & Data Acquisition
Separated compounds enter the detector — DAD, VWD, FLD, or RID. The DAD (G1315A) simultaneously collects full UV-Vis spectra across 1024 diodes (190–950 nm), allowing peak purity assessment and spectral confirmation. ChemStation or OpenLab software processes data and generates compliant reports.
Key Technical Specifications-
| Component / Feature | Specification |
| Pump Type | Quaternary gradient pump (G1311A) |
| Flow Rate Range | 0.001 – 10.000 mL/min |
| Operating Pressure | Up to 400 bar (5,800 psi) |
| Autosampler Injection Range | 0.1 – 100 µL |
| Column Thermostat Range | 10°C below ambient to 80°C |
| Detector Options | DAD, VWD, FLD, RID |
| DAD Wavelength Range | 190 – 950 nm |
| Software | Agilent ChemStation |
| Communication | LAN, GPIB, RS-232 |
Spectrachrome Refurbishment Process
Every Agilent 1100 Series HPLC system supplied by Spectrachrome undergoes a structured, module-by-module reconditioning process. We restore each unit to its original operating specification — not just its appearance.
Our process includes:
• Quaternary pump inspection and seal replacement — pump heads disassembled, seals and pistons inspected, flow accuracy calibrated at multiple set points.
• Autosampler needle and injection valve service — needle, rotor seal, and injection valve inspected and replaced as required to eliminate carry-over.
• Column oven calibration — temperature uniformity verified across the full operating range.
• DAD/VWD lamp performance check — deuterium lamp output measured against specification; replaced if below threshold.
• ChemStation connectivity and method verification — full software communication confirmed with method run and data export validation.
• System suitability testing — retention time reproducibility, peak symmetry, and baseline noise tested using reference standards before dispatch.
Every unit ships with a performance qualification report. You are not buying potential you are buying a verified result.
Ideal Applications for the Agilent 1100 Series HPLC
| Pharmaceutical QC | Method validation, assay of APIs and finished dosage forms, impurity profiling, and dissolution testing under ICH Q2(R1) and USP compliance requirements. |
| Bioanalytical Research | Plasma protein quantification, metabolite profiling, and pharmacokinetic studies requiring high-sensitivity gradient separations with fluorescence or DAD detection. |
| Academic Research | Postgraduate and doctoral research environments needing a dependable gradient HPLC platform for natural product isolation, synthesis monitoring, and method development. |
| Chemical & Industrial QC | Purity testing of chemical intermediates, raw material qualification, and process analytical monitoring across batch production. |
| Environmental Testing | Pesticide residue analysis, PAH determination, and water quality monitoring using validated regulatory methods. |
Why the Agilent 1100 Series Remains a Smart Buy
- The Agilent 1100 HPLC has one of the largest installed bases of any HPLC platform in the world. This means spare parts are readily available, service engineers are familiar with the system, and validated methods written for the 1100 Series can be transferred without revalidation in most cases. For laboratories running established pharmacopoeial or in-house methods, this continuity has direct regulatory and operational value.
- A refurbished Agilent 1100 Series HPLC from Spectrachrome delivers full gradient capability, multi-detector flexibility, and ChemStation software integration at 40–60% below the cost of a comparable new system without sacrificing the analytical performance your laboratory depends on.
What's Included
• Agilent 1100 Quaternary or Binary Pump(as configured)
• G1329A Autosampler
• G1316A Thermostatted Column Compartment.
• G1315A/B DAD or G1314A VWD Detector
• G1322S 4-Channel Degasser
• ChemStation / OpenLab CDS Software + PC
• Cables & Tubing Kit
• Warranty Certificate + Installation Support